Gene description for Flot2
Gene name flotillin 2
Gene symbol Flot2
Other names/aliases Esa
reggie-2
Species Mus musculus
 Experiment description of studies that identified Flot2 in Neuroblastoma cells

1
Experiment ID1631
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

2
Experiment ID1632
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

3
Experiment ID1633
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1634
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID1635
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID1636
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID1637
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

8
Experiment ID1647
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID1648
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID1649
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID1650
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID1651
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID1652
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SampleNeuroblastoma cells
Sample nameN2a
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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